Bio-Techne has patented a method to quantify thiol content in protein mixtures. The method involves conjugating a sample with detection binders, separating protein isoforms, detecting fluorescence signals, and quantifying thiol amounts. The process includes comparing signals with unconjugated isoforms and measuring total-thiol content with a reducing agent. GlobalData’s report on Bio-Techne gives a 360-degree view of the company including its patenting strategy. Buy the report here.
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According to GlobalData’s company profile on Bio-Techne, Peptide pharmacophores was a key innovation area identified from patents. Bio-Techne's grant share as of May 2024 was 37%. Grant share is based on the ratio of number of grants to total number of patents.
Quantifying thiol content in protein isoforms using fluorescence signals
A recently granted patent (Publication Number: US11953436B2) discloses a method for analyzing protein isoforms using a fluorescent detection binder. The method involves generating two sample portions from a sample containing protein isoforms with free thiol groups. A fluorescent detection binder is applied to one portion to bind to the thiol groups without reducing the protein isoforms. The separated protein isoforms are then analyzed to identify and quantify each isoform based on absorbance and fluorescence signals. By measuring the difference in fluorescence signals between the two sample portions, the quantity of free thiol groups associated with each protein isoform can be determined. Additionally, the method allows for the analysis of disulfide groups and drug-antibody conjugates within the protein isoforms.
Furthermore, the patent also describes a system for implementing the method, comprising a processor and memory to receive and analyze fluorescence and absorbance signals associated with separated protein isoforms. The system can identify protein isoforms, calculate the quantity of free thiol groups, and quantify drug-antibody ratios. By utilizing isoelectric focusing to separate protein isoforms, the system can provide detailed information on the composition and characteristics of protein isoforms in a sample. The system's ability to measure absolute quantities of free thiol groups associated with each protein isoform provides valuable insights for various applications, including drug development and protein analysis.
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