Quest Diagnostics. has been granted a patent for methods to detect dihydroxyvitamin D metabolites in samples using mass spectrometry. The patented process includes immunopurification and tandem mass spectrometry techniques to accurately quantify 1,25-dihydroxyvitamin D in serum samples. GlobalData’s report on Quest Diagnostics gives a 360-degree view of the company including its patenting strategy. Buy the report here.
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According to GlobalData’s company profile on Quest Diagnostics, was a key innovation area identified from patents. Quest Diagnostics's grant share as of July 2024 was 55%. Grant share is based on the ratio of number of grants to total number of patents.
The granted patent US12066446B2 outlines a method for quantifying 1,25-dihydroxyvitamin D levels in serum samples using tandem mass spectrometry. The process begins with the addition of a deuterated internal standard to the serum sample, followed by protein precipitation using acetonitrile. The method includes solid phase extraction (SPE) for purifying both the target compound and the internal standard. Subsequently, the 1,25-dihydroxyvitamin D and the internal standard undergo derivatization with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) to form detectable derivatives. The derivatized compounds are then subjected to liquid chromatography, where they are ionized to produce precursor ions with specific mass/charge ratios. The final steps involve fragmenting these precursor ions and determining their amounts, which are then related back to the concentration of 1,25-dihydroxyvitamin D in the serum sample.
Additional claims within the patent specify variations and enhancements to the method. For instance, the deuterated internal standard may consist of deuterated 1,25-dihydroxyvitamin D, and a standard curve can be constructed based on the fragment ions of the internal standard to facilitate quantification. The chromatography technique employed is preferably high-performance liquid chromatography (HPLC), often utilizing a C18 column. The purification process may also include affinity or immunoaffinity separation methods. Furthermore, the detection of fragment ions during mass spectrometry can be achieved through multiple reaction monitoring (MRM), enhancing the method's sensitivity and specificity for accurate measurement of 1,25-dihydroxyvitamin D levels in serum samples.
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