NanoString Technologies has filed a patent for chemical compositions, kits, and apparatuses used in various assays. The patent includes a claim for a probe that consists of a target binding domain linked to a barcode domain, with specific requirements for the nucleotide sequences in each domain. GlobalData’s report on NanoString Technologies gives a 360-degree view of the company including its patenting strategy. Buy the report here.
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According to GlobalData’s company profile on NanoString Technologies, personalized medicine biomarkers was a key innovation area identified from patents. NanoString Technologies's grant share as of September 2023 was 36%. Grant share is based on the ratio of number of grants to total number of patents.
Chemical compositions, kits, and apparatuses for nucleic acid assays
A recently filed patent (Publication Number: US20230257800A1) describes a probe that consists of a target binding domain and a barcode domain. The target binding domain is capable of binding to a target nucleic acid and contains at least eight nucleotides, with at least six nucleotides identifying the target nucleic acid and at least two nucleotides not identifying it. The barcode domain, on the other hand, has a synthetic backbone and includes attachment positions with nucleic acid sequences that can be bound by complementary nucleic acid molecules. The identity of the target nucleic acid bound by the target binding domain is determined by the nucleic acid sequence of the attachment position in the barcode domain.
The synthetic backbone of the probe is made up of single-stranded DNA. Each attachment position in the barcode domain contains one attachment region. The two nucleotides in the target binding domain that do not identify the target nucleic acid follow the six nucleotides that do identify it. The six nucleotides that identify the target nucleic acid are complementary to a portion of the target nucleic acid, while the two nucleotides that do not identify it are not complementary to any part of the target nucleic acid.
The barcode domain can have one or more attachment positions, with each attachment position consisting of between 8 and 20 nucleotides. In some embodiments, each attachment position contains about 20 nucleotides. The probe may also include a complementary nucleic acid molecule that is hybridized to the first attachment region of the first attachment position of the barcode domain. This complementary nucleic acid molecule can have a detectable label, such as a fluorescent label.
The patent also describes a population of probes that includes multiple instances of the probe described above. This population can consist of at least four species of probes, and in some cases, it may contain at least 100 species of probes.
The patent further discloses a method that involves hybridizing a probe to a target nucleic acid, binding a complementary nucleic acid molecule with a detectable label to the attachment position of the barcode domain, and detecting the label. This process is repeated until each attachment position in the barcode domain has been bound by a complementary nucleic acid molecule and the label has been detected. The method can also involve identifying the target nucleic acid based on the detected labels.
Additionally, the patent describes a method that includes hybridizing a plurality of probes to a plurality of target nucleic acids in a sample, binding complementary nucleic acid molecules with detectable labels to the attachment positions of the barcode domains of the hybridized probes, and detecting the labels. This process is repeated until each attachment position in the barcode domains of the hybridized probes has been bound by a complementary nucleic acid molecule and the label has been detected. The method can also involve identifying the target nucleic acids based on the detected labels. This method can be applied to tissue samples.
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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies
